Efficient synthesis of human type alpha transforming growth factor: its physical and biological characterization.
- 1 November 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (21) , 8082-8086
- https://doi.org/10.1073/pnas.83.21.8082
Abstract
Human transforming growth factor type .alpha. (TGF-.alpha.) was synthesized by a stepwise solid-phase method with an overall yield of 26%. Synthetic TGF-.alpha., consisting of 50 amino acid residues deduced from a cDNA precursor sequence, was purified in a single HPLC step. The homogeneity and primary structure were confirmed by several criteria including Edman degradation and mass spectrometry. Synthetic TGF-.alpha. was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-.alpha. stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-.alpha. showed similar immunoreactivity when compared with rat TGF-.alpha.. Thus, the 50-amino acid TGF-.alpha. is likely to be the bioactive principle produced and secreted by tumor cell lines.This publication has 24 references indexed in Scilit:
- A synthetic approach to structure-function relationships in the murine epidermal growth factor molecule.Proceedings of the National Academy of Sciences, 1986
- Automated Chemical Synthesis of a Protein Growth Factor for Hemopoietic Cells, Interleukin-3Science, 1986
- Synthesis of biologically active rat transforming growth factor INature, 1984
- Human transforming growth factor-α: Precursor structure and expression in E. coliPublished by Elsevier ,1984
- Down-regulation of epidermal growth factor receptor correlates with plasminogen activator activity in human A431 epidermoid carcinoma cells.Proceedings of the National Academy of Sciences, 1983
- Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reactionAnalytical Biochemistry, 1981
- Kirsten murine sarcoma virus transformed cell lines and a spontaneously transformed rat cell‐line produce transforming factorsJournal of Cellular Physiology, 1980
- Growth factors from murine sarcoma virus-transformed cells.Proceedings of the National Academy of Sciences, 1978
- Epidermal growth factor, like phorbol esters, induces plasminogen activator in HeLa cellsNature, 1978
- Californium-252 Plasma Desorption Mass SpectroscopyScience, 1976