SOME CYTOLOGICAL AND PATHOGENIC PROPERTIES OF SPHEROPLASTS OFCANDIDA ALBICANS

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Abstract
Kobayashi, GeorgeS. (Tulane University, New Orleans, La.),Lorraine Friedman, and Judith F. Kofroth. Some cytological and pathogenic properties of spheroplasts ofCandida albicans. J. Bacteriol.88:795–801. 1964.—Spheroplasts ofCandida albicanswere prepared by use of an enzymatic mixture from the digestive tract of the snailHelix pomatia. Untreated cells exhibited well-defined cell walls, whereas such structures were absent from spheroplasts. The intravenous inoculation of either spheroplasts or intact cells into rabbits produced a fever which was apparent within 30 min, the “immediate” fever response characteristic of microbial endotoxin. Cell-wall fragments of enzyme-treated cells did not induce a convincing pyrogenic response. When the inoculum was viable, body temperatures did not return to normal but remained elevated until death of the animal 1 or more days later, exhibiting the “delayed” fever of infection. The gross pathological picture in animals succumbing to infection by viable spheroplasts was similar to that obtained with untreated yeast cells.

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