DETERMINATION OF THE DIRECTION OF TRANSCRIPTION OF BACTERIOPHAGE-T4 GENES BY HEAT INDUCTION OF TRANSCRIPTION UNDER CONTROL OF PL PROMOTER OF RECOMBINANT PLASMIDS - 2 DIRECTIONS IN THE REGION OF GENES 25-29
- 1 January 1988
- journal article
- research article
- Vol. 24 (1) , 42-52
Abstract
The bacterial strains with recombinant plasmids constructed on the basis of vector plasmid pSCC31 and containing BlII fragment of bacteriophage T4 DNA with genes 25-29 have been used in this study. Restriction analysis and subcloning demonstrated that in the case of the recombinant plasmid pRL705, the phage DNA fragment had right orientation for phage late genes transcription, while the opposite one in the plasmid pRL707. Heat induction of plasmids transcription under control of pL promoter led to the substantial change in phage burst size and the production of recombinants, as shown in complemenation experiments, the changes in the burst size being dependent both on the host strain and the amber mutant used. On the basis of these data, the conclusion has been drawn that the genes 26 and 25, in contrast to genes 51, 27, 28 and 29, are being transcribed in the counter-clockwise direction on the genomic map, i.e. in the direction of transcription of T4 early genes.This publication has 7 references indexed in Scilit:
- MOLECULAR-CLONING OF SOME BGIII FRAGMENTS OF BACTERIOPHAGE-T4 DNA IN THE VECTOR PLASMID PSCC311988
- Nucleotide sequence of bacteriophage T4 uvsY geneVirology, 1985
- THE INVESTIGATION OF THE TRANSMISSION OF BACTERIOPHAGE-T4 AMBER MUTANTS .2. THE TEMPERATURE SENSITIVITY OF MULTIPLICATION OF GENE-26 AMBER MUTANTS IN ESCHERICHIA-COLI-B CELLS AND THE LACK OF SUCH SENSITIVITY IN THE CASE OF GENE-331983
- A T4 DNA fragment containing genes for the baseplate central plug: Endonuclease restriction, gene expression and cell wall changesMolecular Genetics and Genomics, 1982
- Bacteriophage T4 gene transcription studied by hybridization to cloned restriction fragmentsJournal of Molecular Biology, 1980
- Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genesMolecular Genetics and Genomics, 1977
- CONNECTION BETWEEN PROTEIN SYNTHESIS AND REGULATION OF MESSENGER RNAS FORMATION IN E. COLI B CELLS UPON DEVELOPMENT OF T2-PHAGE1963