An HPLC Method for the Determination of Theophylline and Its Metabolites in Serum and Urine

1 July 1984

journal article
research article
Published by Taylor & Francis in Journal of Liquid Chromatography

Vol. 7 (8) , 1593-1608
https://doi.org/10.1080/01483918408074069

Abstract

A urine and a serum assay have been developed to quantitate theophylline and its major metabolites:1,3-dimethyluric acid, 3-methylxanthine and 1-methyluric acid. Reverse phase chromatography follows a serum acetone extraction procedure and a urine anion exchange clean-up procedure. Lower limits of sensitivity are 0.04 μg/ml for serum metabolites and 1 μg/ml for urine metabolites. Both assays are free of interference from endogenous substances. These assays have been tested successfully in pharmacokinetic and metabolic studies of theophylline.

Keywords

This publication has 13 references indexed in Scilit:

Secondary metabolism of theophylline biotransformation products in man‐ route of formation of 1‐methyluric acid.
British Journal of Clinical Pharmacology, 1983
Non-linear elimination processes of theophylline
European Journal of Clinical Pharmacology, 1983
An Automated HPLC Assay for Simultaneous Quantitation of Methylated Xanthines and Uric Acids in Urine
Journal of Chromatographic Science, 1982
Nonlinear theophylline elimination
Clinical Pharmacology & Therapeutics, 1982
Measurement of excretion characteristics of theophylline and its major metabolites
European Journal of Clinical Pharmacology, 1981
Simultaneous determination of theophylline and its major metabolites in urine by reversed-phase ion-pair high-performance liquid chromatography
Journal of Chromatography B: Biomedical Sciences and Applications, 1980
Separation of Xanthine Derivates by High Pressure Liquid Chromatography and Application to Plasma Analysis
Journal of Liquid Chromatography, 1980
Metabolism of theophylline to caffeine in premature newborn infants
The Journal of Pediatrics, 1979
Theophylline Estimation — a Comparative Evaluation of a Gas Chromatographic Method and a High-Performance Liquid Chromatographic Method
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1979
Micro-scale method for theophylline in body fluids by reversed-phase, high-pressure liquid chromatography.
Clinical Chemistry, 1977