Zur Frage des Vorkommens von Butyryicholin im Rindergehirn

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Abstract
Contrary to statements by Holtz and Schumann, no butyryl-choline could be detected in beef brain when ethanol, propanol and acetone extracts were submitted to paper chromatographic separation. In its place another substance was obtained which was not identified but on the basis of its pharmacological properties alone could be mistaken for the ester. n-Butyrylcholine was isolated when beef brain was minced and incubated with eserine for 2 hours at 37[degree] and the tissue bases purified by reineckate precipitation from ethanol and trichlor-acetic acid extracts. After preliminary paper chromatographic separation, acetyl- and n-butyrylcholine were obtained pure from high-voltage pherograms. Their acid components were characterized as the Fe[image]-hydroxamate complexes, the choline as dipicrylamine derivative after alkaline hydrolysis and the n-butyrylcholine by micro-determination of the melting point of its chloroaurate. When allowance is made for losses which occur in the working up, the n-butyrylcholine content of pre-treated brain is found to be approximately 40 gamma/kg. The question as to whether n-butyrylcholine is an endogenous substance remains open.