Zur Identifizierung von Cholinestern in biologischem Material, insbesondere von Acetylcholin in Bienenfuttersäften

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Abstract
Royal jelly of bees was precipitated with ethanol and then extracted with trichloracetic acid. On paper chromatography two substances with the chemical and pharmacological properties of acetyl choline were detected. As the two substances appeared to be very similar, they were further examined. The substances were subjected to paper electrophoresis (pH 4.6); they migrated as one spot. The fatty acid components were then examined by paper chromatography (solvent n-butanol-n-propanol-water, 20:7:3) of the ferric complexes of their hydroxamic acids, a method which was extremely sensitive; it was capable of detecting 0.002[mu]M. Using this method, only one fatty acid, acetic acid, was found. It was thus concluded that the chromatographic behavior of acetyl choline in n-butanol-ethanol-acetic acid-water, 8:2:1:3 is anomalous.