A Method for the Automated Screening for Hepatitis B Surface Antigen

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Abstract
A reverse passive hemagglutination (RPHA-G) technique for the automated screening of hepatitis B surface antigen (HBsAg) [in humans] on the Groupamatic 360 (Kontron International, Zurich, Switzerland) was developed. It is more sensitive than manual RPHA (RPHA-M) and nearly as sensitive as radioimmunoassay (RIA); it detects 6 ng/ml of HBsAg, compared to 3 ng/ml by RIA and 25 ng/ml by RPHA-M. Although it is slightly inferior to the other 2 methods in respect to specificity, the incidence of false-positive reactions was only 0.34%. The test can also be performed manually without the Groupamatic.