Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.
- 1 July 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (7) , 2734-2738
- https://doi.org/10.1073/pnas.74.7.2734
Abstract
The 7-methylguanylic acid residue confronting the 59-terminal nucleotide of mRNA through two pyrophosphate linkages was completely removed by tobacco pyrophosphatase from mRNAs of cytoplasmic polyhedrosis virus, tobacco mosaic virus (viral RNA), and globin without any scission in the inner part of the RNA chain. Protein synthesis ability in a wheat germ cell-free system was lost after this treatment of all three kinds of mRNA. The initiation complexes for protein synthesis of these three RNAs were not obtained after using tobacco phosphodiesterase-treated mRNA. On incubation of mRNA in a wheat germ extract, the mRNA lacking m7G was quickly degraded from the 59 terminus in an exonucleolytic way, whereas the intact mRNA remained stable. These results show that one of the confronting nucleotide structure9s functions is to stabilize the mRNA, to prevent its degradation.Keywords
This publication has 27 references indexed in Scilit:
- A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.Proceedings of the National Academy of Sciences, 1976
- The process of formation of the 5′-terminal modified structure in messenger RNA of cytoplasmic polyhedrosis virusFEBS Letters, 1976
- Inhibition of the transition of a 40S ribosome–Met-tRNAiMet complex to an 80S ribosome–Met-tRNAiMet complex by 7-methylguanosine-5′-phosphateNature, 1976
- The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.Proceedings of the National Academy of Sciences, 1976
- 5'-terminal structure of poliovirus polyribosomal RNA is pUp.Proceedings of the National Academy of Sciences, 1976
- Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate.Proceedings of the National Academy of Sciences, 1976
- 5′-Terminal 7-methylguanosine in eukaryotic mRNA is required for translationNature, 1975
- Methylation-dependent translation of viral messenger RNAs in vitro.Proceedings of the National Academy of Sciences, 1975
- A blocked structure at the 5′ terminus of mRNA from cytoplasmic polyhedrosis virusNature, 1975
- Purification of viral RNA by means of bentoniteVirology, 1961