Determination Of m-Chlorophenyl-Piperazine in Plasma by High-Performance Liquid Chromatography with Coulometric Detection

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Abstract
M-Chlorophenylpiperazine (mCPP) and the internal standard, l-(2-pyrimidinyl)piperazine (1PP) are extracted from base matrix using CN sorbent extraction columns. Chromatography and detection are performed using isocratic reverse-phase high performance liquid chromatography (HPLC) with a CN column and coulometric end-point detection. The standard curve was linear over the range 0–25ng/ml of plasma. The lower limit of quantitation is 0.2ng/ml of mCPP from 1ml of plasma. The reproducibility (CV) of the method over the range of the standard curve varied from 3–8.5%. The inter-assay CV was 5.9%. Recovery averaged 92.5 ± 8.6%. Plasma profiles of mCPP following varying doses of nefazodone HC1 are given.