Differentiated analysis of the secondary structure of hydrophilic and hydrophobic regions in α- and β-subunits of Na+,K+-ATPase by Raman spectroscopy
- 25 January 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 227 (2) , 235-239
- https://doi.org/10.1016/0014-5793(88)80905-0
Abstract
Raman spectra of active Na+,K+-ATPase from pig kidney and membrane-bound products of its two-stage trypsinolysis, including α-subunit hydrophobic regions as well as the intact β-subunit and hydrophobic regions of α- and β-subunits, were measured to calculate the secondary structure of hydrophilic and hydrophobic regions of the enzyme. Consequent comparison demonstrated unambiguously that (i) membrane-bound hydrophobic parts of polypeptide chains of Na+,K+-ATPase subunits are in the α-helical conformation; (ii) essential contents of the α-helix as well as β-sheet are estimated to form the hydrophilic (mainly cytoplasmic) domain of the Na+,K+-ATPase α-subunit; (iii) the exoplasmic hydrophilic domain of the β-subunit is shown to include several antiparallel β-pleated sheets and a small amount of the α-helix and unordered conformations. The model of the secondary structure organization of hydrophilic domains as well as 8 hydrophobic transmembrane segments of the enzyme molecule was proposed on the basis of experimental results and predictional calculationsKeywords
This publication has 21 references indexed in Scilit:
- Detailed structural analysis of exposed domains of membrane‐bound Na+, K+‐ATPase A model of transmembrane arrangementFEBS Letters, 1987
- Pig kidney Na+,K+‐ATPaseFEBS Letters, 1986
- Amino-acid sequence of the β-subunit of the (Na+ + K+)ATPase deduced from a cDNANature, 1986
- Primary structure of the β‐subunit of Torpedo californica (Na+ + K+)‐ATPase deduced from the cDNA sequenceFEBS Letters, 1986
- Structure of purple membrane from halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolutionUltramicroscopy, 1986
- Three‐dimensional structure of (Na+ + K+)‐ATPase revealed by electron microscopy of two‐dimensional crystalsFEBS Letters, 1985
- Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNANature, 1985
- Primary structure of the α-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequenceNature, 1985
- Asymmetric orientation of amino groups in the α-subunit and the β-subunit of (Na+ + K+)-ATPase in tight right-side-out vesicles of basolateral membranes from outer medullaBiochimica et Biophysica Acta (BBA) - Biomembranes, 1985
- The Na+, K+-Transporting Adenosine TriphosphatasePublished by Springer Nature ,1985