The right end of MudI(Ap,lac)
- 1 December 1989
- journal article
- research article
- Published by Springer Nature in Archiv für Mikrobiologie
- Vol. 153 (1) , 1-6
- https://doi.org/10.1007/bf00277532
Abstract
Stable derivatives of the bacteriophage MudI(Ap,lac) were used to generate operon fusions in S. typhimurium which exhibit a sectoring phenotype with respect to lacZ expression. The Lac- to Lac+ conversion was shown to be the result of small deletions involving the right end of the MudI element. DNA sequence analysis of several different fusions revealed that this end of MudI(Ap,lac) contains an assymetric inverted repeat of the attR site found in the wild-type Mu phage. A model is presented which explains how such a structure was formed in the construction of MudI(Ap,lac). In addition, this model explains the observed deletion formation and the Lac- to Lac+ conversion in the sectoring fusions.This publication has 16 references indexed in Scilit:
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