Planned immunization of human volunteers with HLA‐DR incompatible lymphocytes

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Abstract
Aiming at the production of anti HLA‐DR test sera, eight healthy human volunteers were immunized by repeated intradermal injections of lymphocytes which were selected to be incompatible for one HLA‐DR antigen, and matched as well as possible for HLA‐A,‐B,‐C antigens. One out of 3 recipients immunized exclusively against HLA‐DR produced lyrnphocytotoxic HLA‐DR antibodies. The remaining 5 recipients were immunized against 1 or more HLA‐A,‐B,‐C antigens in addition to one HLA‐DR antigen. After 3 immunizations, 3 of these reacted with strong HLA‐A or ‐B antibody production; however, only one showed a parallel anti HLA‐DR antibody response detectable by complement dependent lymphocytotoxicity.Testing of the recipient sera in the antibody dependent cell‐mediated cytotoxicity (ADCC) assay revealed that 6 of the 8 recipients did react early to the immunizations with HLA specific antibody production. However, in spite of repeated booster injections it was not possible to obtain more than the above‐mentioned 2 sera with HLA‐DR antibodies strong enough to react in the lymphocytotoxicity microtechique.