Simultaneous Molecular and Hypoxia Imaging of Brain Tumors In Vivo Using Spectroscopic Photoacoustic Tomography
Top Cited Papers
- 12 February 2008
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in Proceedings of the IEEE
- Vol. 96 (3) , 481-489
- https://doi.org/10.1109/jproc.2007.913515
Abstract
Noninvasive molecular and functional imaging in vivo is promising for detecting and monitoring various physiological conditions in animals and ultimately humans. To this end, we present a novel noninvasive technology, spectroscopic photoacoustic tomography (SPAT), which offers both strong optical absorption contrast and high ultrasonic spatial resolution. Optical contrast allows spectroscopic separation of signal contributions from multiple optical absorbers (e.g., oxyhemoglobin, deoxyhemoglobin, and a molecular contrast agent), thus enabling simultaneous molecular and functional imaging. SPAT successfully imaged with high resolution the distribution of a molecular contrast agent targeting integrin overexpressed in human U87 glioblastomas in nude mouse brains. Simultaneously, SPAT also imaged the hemoglobin oxygen saturation and the total hemoglobin concentration of the vasculature, which revealed hypoxia in tumor neovasculature. Therefore, SPAT can potentially lead to better understanding of the interrelationships between hemodynamics and specific biomarkers associated with tumor progression.Keywords
This publication has 40 references indexed in Scilit:
- In vitromeasurements of absolute blood oxygen saturation using pulsed near-infrared photoacoustic spectroscopy: accuracy and resolutionPhysics in Medicine & Biology, 2005
- Deeply penetrating photoacoustic tomography in biological tissues enhanced with an optical contrast agentOptics Letters, 2005
- Ferritin as an Endogenous MRI Reporter for Noninvasive Imaging of Gene Expression in C6 Glioma TumorsNeoplasia, 2005
- Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia developmentJournal of Biomedical Optics, 2005
- Multiple-bandwidth photoacoustic tomographyPhysics in Medicine & Biology, 2004
- Ultrasound‐Mediated Biophotonic Imaging: A Review of Acousto‐Optical Tomography and Photo‐Acoustic TomographyDisease Markers, 2004
- Time-domain reconstruction algorithms and numerical simulations for thermoacoustic tomography in various geometriesIEEE Transactions on Biomedical Engineering, 2003
- An implantable guide-screw system for brain tumor studies in small animalsJournal of Neurosurgery, 2000
- Requirement of Vascular Integrin α v β 3 for AngiogenesisScience, 1994
- Involvement of integrin alpha V gene expression in human melanoma tumorigenicity.Journal of Clinical Investigation, 1992