Differential Ion Mobility Separations of Peptides with Resolving Power Exceeding 50
- 25 November 2009
- journal article
- letter
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 82 (1) , 32-35
- https://doi.org/10.1021/ac902133n
Abstract
Differential ion mobility spectrometry (IMS) or field asymmetric waveform IMS (FAIMS) separates gas-phase ions by mobility differences with respect to the electric field intensity. A major emerging FAIMS application is the fractionation of proteolytic digests. Using a planar FAIMS unit with helium/nitrogen mixtures, we have increased FAIMS resolving powers for peptide analyses from the prior maximum of ∼20−30 to ∼50−70. The resolution improved nearly 3-fold, allowing, in particular, separation of previously unresolved conformers.This publication has 25 references indexed in Scilit:
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