Thrombopoiesis and Megakaryocyte Colony Stimulating Factor in the Urine of Patients with Aplastic Anaemia

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Abstract
Summary The urinary extracts from patients with aplastic anaemia and from healthy donors were investigated in vivo and in vitro for their ability to stimulate megakaryopoiesis and platelet production. There was a significantly higher concentration of thrombopoiesis stimulating factor (TSF) and megakaryocyte colony stimulating factor (MEG‐CSF) in the urine from patients with aplastic anaemia than in that from healthy donors. Neuraminidase treatment did not affect the thrombopoietic activity of TSF, whereas coexisting erythropoictin (EPO) in the extract lost its activity in vivo. These findings suggest that TSF and/or MEG‐CSF seems to be different from EPO and that the urine from aplastic anaemia patients would be a good source of TSF and MEG‐CSF for purification and characterization.