Nucleosome reconstitution on plasmid-inserted poly(dA) . poly(dT).
Open Access
- 1 February 1982
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 1 (2) , 173-179
- https://doi.org/10.1002/j.1460-2075.1982.tb01143.x
Abstract
Chromatin was reconstituted from core histones and recombinant plasmid DNAs carrying poly(dA) . poly(dT) inserts of various lengths. A 97‐bp insert was found to occupy discrete and regularly‐spaced positions on the edges of the nucleosome. This insert cannot, however, be entirely included due to a block in the center of the particle. In contrast, nucleosomes reconstitute on a shorter 20‐bp insert. In this case, the insert shows a marked preference for the edges of the particle. Possible structural and physiological implications of these observations are discussed.This publication has 28 references indexed in Scilit:
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