Liver xanthine oxidase

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Abstract
The self-respiration of minced ox liver was found to be due mainly to purine-base oxidation and was controlled by the rate of formation of the latter by nucleosidase action. Methods are given for the preparation of xanthine oxidase from liver. The kinetics of aldehyde and xanthine oxidation were studied manometrically, making a correction for the oxidation of aldehydes in the presence of alkali. The Km for acetaldehyde was 0.005 M. The enzyme was destroyed by aldehydes. It contains catalase and the cyanide inhibition of xanthine oxidation is due to destruction of this catalase. Oxidation of aldehydes by both minced liver and enzyme preparations was inhibited by xanthine and other purine derivatives.