Simultaneous Determination of Neutral Sugars and Uronic Acids in Hydrocolloids

Publisher Website
Google Scholar
Abstract
An improved gas‐chromatographic method for the simultaneous determination of neutral sugars and uronic acids in hydrocolloids or industrial gums was developed. The procedure involved methanolysis with 0.5M methanolic HCl followed by trimethylsilylation. This method was simplified over other derivatization methods to make it suitable for the routine analysis of hydrocolloids or other polysaccharides which contain uronic acids. The usefulness of this method was demonstrated by analysis of commercially available hydrocolloids. The described method was sensitive enough to identify some of the minor sugar components which were not easily identified by other derivatization methods.