An improved isolated heart preparation for external assessment of myocardial metabolism
- 1 April 1979
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 236 (4) , H644-H661
- https://doi.org/10.1152/ajpheart.1979.236.4.h644
Abstract
To facilitate characterization of myocardial metabolism with the use of positron-emitting tracers, we developed an isolated heart preparation designed to simulate physiological levels of flow and substrate extraction. Isovolumically beating hearts were perfused retrograde at 60 mmHg with modified non-recirculating Krebs-Henseleit (KH) solution with or without washed sheep red blood cells at a hematocrit of 25 or 40 (KH-RBC25, KH-RBC40). Left ventricular (LV) systolic pressure averaged 63 mmHg with KH alone, 78 with KH-RBC25, and 98 with KH-RBC40. LV dP/dt, pressure-time index, and oxygen consumption increased in an analogous fashion. Fatty acid extraction increased from 1.5% with KH to 11.8% in hearts perfused with KH-RBC40, although net uptake was not increased markedly because of the associated differences in flow (4.6 ml.g-1.min-1 with KH and 1.5 with KH-RBC40). Hearts perfused with erythrocyte-rich media exhibited functional and metabolic stability and less edema. The preparation avoids intrinsic limitations encountered with buffer-perfused hearts and should prove useful for characterization of cardiac metabolism with radiolabeled tracers relevant to positron emission transaxial tomography.This publication has 13 references indexed in Scilit:
- External detection and visualization of myocardial ischemia with 11C-substrates in vitro and in vivo.Circulation Research, 1976
- Use of radioactive microspheres to assess distribution of cardiac output in rabbitsAmerican Journal of Physiology-Legacy Content, 1968
- Apparatus for study of ventricular function and metabolism in the isolated perfused rat heart.Journal of Applied Physiology, 1967
- Effect of pressure development on oxygen consumption by isolated rat heartAmerican Journal of Physiology-Legacy Content, 1967
- Removal of Fatty Acids from Serum Albumin by Charcoal TreatmentJournal of Biological Chemistry, 1967
- Colorimetric ultramicro method for the determination of free fatty acidsJournal of Lipid Research, 1965
- Equations for Measuring Blood Flow by External Monitoring of RadioisotopesCirculation Research, 1965
- Aggregation of Blood Cells by 5-Hydroxytryptamine (Serotonin)Circulation Research, 1963
- MYOCARDIAL METABOLISM OF FATTY ACIDS*Journal of Clinical Investigation, 1960
- An isolated mammalian heart preparation capable of performing work for prolonged periodsThe Journal of Physiology, 1933