Effect of Thiocyanate, Perchlorate and Other Anions on Plasma Protein-Thyroid Hormone Interaction in Vitro

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Abstract
Two methods we''re used for the study: the uptake of labeled thyroxine (131I-T4) and triiodothyronine (131I-T3) by muscle and electrophoresis of plasma containing 131I-T4. Increases in the anion concentration and the uptake by muscle were parallel in the presence of dilute plasma, while, in the absence of plasma, there was no anion effect upon muscle uptake. The increase in muscle uptake paralleled the known activity of each thyroid blocking anion (ClO4-> SCN->BF4 >NO3> ClO3). Other anions (Cl-, BrO3- and MnO4-), known to be uneffective in blocking thyroid iodide transport, failed to increase the muscle uptake of 131I-T4 in the presence of plasma. Electrophoresis on acrylamide and starch gel indicated an in vitro displacement of 131I-T4 from prealbumin-albumin area in the presence of thyroid blocking anions.