Electrophoretic Investigation of Fibrin and Fibrinogen Dissolved in Urea Solutions.

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Abstract
The electrophoretic mobility curves of fibrin and fibrinogen are approx. parallel over the pH range of 4.5 to 12. Their viscosity curves differ considerably, but this is not considered responsible for these slight differences in mobilities. In the presence of 20% urea, the ionic strength has little influence on the electrophoretic mobilities. The electrophoretic mobility curve of fibrin is always below that of fibrinogen. Between pH 5.3 and 6.8 the 2 curves are very close together, but at more basic pH they separate, rejoining again at very alkaline values. Fibrin showed sharper boundaries than fibrinogen. Urea does not affect appreciably the electrophoretic mobility of fibrinogen. The isoelectric point of fibrinogen in 20% urea is at pH 5.5; that of fibrin at pH 5.6.