Advanced multiplexed analysis with the FlowMetrixTM system
Open Access
- 1 September 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 43 (9) , 1749-1756
- https://doi.org/10.1093/clinchem/43.9.1749
Abstract
The FlowMetrixTM System is a multiplexed data acquisition and analysis platform for flow cytometric analysis of microsphere-based assays that performs simultaneous measurement of up to 64 different analytes. The system consists of 64 distinct sets of fluorescent microspheres and a standard benchtop flow cytometer interfaced with a personal computer containing a digital signal processing board and Windows95®-based software. Individual sets of microspheres can be modified with reactive components such as antigens, antibodies, or oligonucleotides, and then mixed to form a multiplexed assay set. The digital signal-processing hardware and Windows95-based software provide complete control of the flow cytometer and perform real-time data processing, allowing multiple independent reactions to be analyzed simultaneously. The system has been used to perform qualitative and quantitative immunoassays for multiple serum proteins in both capture and competitive inhibition assay formats. The system has also been used to perform DNA sequence analysis by multiplexed competitive hybridization with 16 different sequence-specific oligonucleotide probes.Keywords
This publication has 14 references indexed in Scilit:
- Chapter 33 Flow Microsphere Immunoassay for the Quantitative and Simultaneous Detection of Multiple Soluble AnalytesPublished by Elsevier ,1994
- Reliable Hybridization of Oligonucleotides as Short as Six NucleotidesDNA and Cell Biology, 1990
- Early detection of antibodies against rDNA-produced HIV proteins with a flow cytometric assayBlood, 1989
- HLA-DQβ gene contributes to susceptibility and resistance to insulin-dependent diabetes mellitusNature, 1987
- Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairsNucleic Acids Research, 1987
- A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotidesGene, 1986
- Three different mutations in codon 61 of the human N-rasgene detected by synthetic oligonucleotide hybridizationNucleic Acids Research, 1984
- Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.Proceedings of the National Academy of Sciences, 1983
- Hybridization of synthetic oligodeoxyribonucleotides to ΦX174 DNA: the effect of single base pair mismatchNucleic Acids Research, 1979
- Quantitative Single Cell Analysis and SortingScience, 1977