Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells.
- 1 November 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (21) , 6522-6526
- https://doi.org/10.1073/pnas.79.21.6522
Abstract
A number of abbreviated dihydrofolate reductase (DHFR) genes were constructed by using cloned mouse genomic and c[complementary]DNA sequences. These genes contain 1.0 kilobase of 5'' flanking genomic sequence and varying portions of the 3'' non-coding region. Two of the genes contain the first 2 introns of the DHFR gene; the other 3 lack introns. Transfection of DHFR-deficient Chinese hamster ovary cells with any of these constructed genes results in cells with the DHFR+ phenotype. Treatment of the transfectants with methotrexate, a folate antagonist, leads to the emergence of methotrexate-resistant colonies which amplified the transfected genes.This publication has 24 references indexed in Scilit:
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