5-day storage of single-donor platelets obtained using a blood cell separator

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Abstract
Platelets were collected from normal donors via a blood cell separator (Fenwal CS-3000). Platelets were stored initially in two separate 1000-ml bags (average count per bag, 2.3 .+-. 0.5 .times. 1011) in 100 ml of autologous plasma for 5 days. Little change in platelet count was noted after 5 days of storage; however, the white cell count fell from 5.1 .+-. 1.7 .times. 109 at Time 0 to 3.4 .+-. 2.3 .times. 109 per 1 at Day five. The initial lactate values were 32 .+-. 11 mg per dl and rose to 165 .+-. 28 mg per/dl by 5 days. Platelet aggregation was impaired both by the collection procedure and during storage: whereas the response to ADP of the donors'' platelets before the procedure was 100 percent, samples taken from the product immediately after collection had only a 45 percent response, which fell to 12 percent by Day 5. Aggregation using epinephrine was similarly affected, with a 75 percent response after collection and 0 percent response by Day 5. The plasma .beta.-thromboglobulin (.beta.-TG) level was high, both after collection (5.0 .mu.g/ml at Time 0) and after storage (11.0 .mu.g/ml), indicating a considerable effect of collection on platelet alpha granule release. In vivo recovery of these platelets was very good at 67 .+-. 6 percent, with an average survival of 7.3 .+-. 1.4 days (multiple hit; n = 4). The addition of 100 ml of autologous plasma to one bag of platelets before storage did not change the results significantly, except for the aggregation response to ADP immediately after collection (76% with plasma; 45% with plasma; 45% without; p > 0.05); improvement was still apparent after 5 days of storage. The concentration of lactate and .beta.-TG was reduced by one-half by 5 days, but this value simply reflects the doubled plasma volume. The in vivo platelet recovery of autologous platelets in four normal individuals after 5 days of storage was extremely good, 78 .+-. 10 percent; survival was 6.1 .+-. 0.9 days.