Recovery of Totipotent Cells and Plantlet Production from Daylily Protoplasts

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Abstract
The release of protoplasts by means of enzymes from a totipotent cell suspension of a diploid daylily (Hemerocallis cv. ‘Autumn Blaze’), their collection and distribution in plastic culture vessels and their subsequent regeneration is described. Attention is given to aggregation and to the different forms of growth during the initial stages of culture. The methods for the subsequent multiplication of the regenerated cells into morphogenetically competent compact clusters and the manipulation of these clusters to yield embryo-plantlets are also outlined. The implications of all this in terms of potential for the genetic modification of daylily is discussed.