The Partial Purification of Staphylocoagulase and the Effect of Certain Presumptive Inhibitors upon Its Plasma-coagulating Action

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Abstract
Concentrated staphyloco-agulase is prepd. from the supernatants of autoclaved cultures of Staphylococcus aureus, grown for 1 wk. in beef heart tryptic digest broth (pH 7.6). Supernatant is adjusted to pH 4 with HCl, washed with acetate buffer (pH 4) and the ppt. redissolved at pH 7.5. Pptn. is repeated once. The final product is dried from the frozen state in vacuum. Final activity, in terms of units per g. N is about 1000 times the original. Activity was tested by the serial dilution method, with a substrate of fresh human plasma. Substances tested for inhibition were mixed with plasma and the mixture used for titration of staphylocoagulase, using the same normal plasma as control. Activity is inhibited by streptomycin, propylene glycol, and NaN3. No inhibition was caused by penicillin, zephiran, bacitracin, tyro-thricin, gramicidin, tyrocidin, hydrazine, sulfathiazole, or sulfadiazine.