GENETIC STUDY OF LYSINE BIOSYNTHESIS IN YEAST

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Abstract
The genetical analysis of lysine-requiring mutants revealed that a -aminoadipic acid (A A A) is not an obligatory intermediate for lysine biosynthesis in yeast. AAA may contribute to the biosynthesis of lysine by conversion into a hypothetical compound X which is an unspecified intermediate directly in the lysine pathway. Mutations which block the conversion from AAA to compound X do not necessarily block lysine biosynthesis. By exploiting tetrad analysis it has been shown that in Saccharomyces, mutations occurring at lysine loci 1, 2, 5, 6, 9, 13 and 14 may block the steps after compound X in the lysine pathway, and that mutations occurring at lysine loci 4, 7, 8, 11 and 12 may block the steps prior to compound X.