Determinations of Lactose in Mammary Gland and in Milk

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Abstract
A chromatographic method for the separation of monosaccharides from larger molecular weight carbohydrates was adapted to the analysis of lactose. Chromatographic separation was effected on a charcoal-Celite column; water was used to elute monosaccharides, followed by 80% EtOH which eluted lactose. The orcinol reaction was used to determine the amount of lactose present. Polysaccharides such as glycogen are retained on the chromatographic column. Recovery experiments with mixtures of ribose, lactose, and glycogen, alone or added to milk and mammary gland suspensions, demonstrated the effectiveness of the procedure.