High-Performance Liquid Chromatographic Micro-Assay for Cefoperazone in Human Plasma, Urine and CSF

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Abstract
A high performance liquid chromatographic method is described for the determination of cefoperazone in plasma, urine and CSF samples. The procedure employs reversed-phase chromatography, using hydrochlorothiazide as an internal standard. The assay only requires 100 μL of sample, with direct injection of diluted urine or CSF, or protein precipitated plasma. Stability studies indicate the need for careful attention to processing and storage conditions of the biologic material. The method is specific for cefoperazone in the presence of kanamycin, tobramycin, gentamicin, ampicillin and penicillin G. The method has been successfully employed in the assay of over 600 samples obtained from human clinical trials.