Characterization and nucleotide sequence of a chicken gene encoding an opal suppressor tRNA and its flanking DNA segments.

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Abstract
A naturally occurring opal suppressor serine t[transfer]RNA were purified from chicken liver and used as a probe to isolate the corresponding gene from a library of chicken DNA in bacteriophage .lambda.. This minor RNA is encoded by a single-copy gene that is not part of a tRNA gene cluster. DNA sequence analysis of the gene and its flanking DNA segments shows that the gene is encoded in an 87-base-pair segment without intervening sequences and specifies a tRNA that reads the termination codon UGA. This gene has additional nucleotides in the 5'' internal promoter region but has a normal 3'' internal promoter sequence and the usual termination signal.