Synechocystis PCC 6803 Contains a Single Gene for the [beta] Subunit of Tryptophan Synthase with Strong Homology to the trpB Genes of Arabidopsis and Maize (Zea mays L.)
- 1 February 1994
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 104 (2) , 461-466
- https://doi.org/10.1104/pp.104.2.461
Abstract
We report the sequence of the trpB gene of the cyanobacterium Synechocystis sp. PCC 6803. This gene was cloned from a plasmid library by functional complementation of a trpB mutant of Escherichia coli K-12. Among the known trpB sequences, the Synechocystis gene bears the greatest homology to the duplicated trpB genes of Arabidopsis thaliana and Zea mays. Southern and northern blotting analyses suggest that Synechocystis contains only a single trpB gene. In contrast to all other prokaryotes, Synechocystis has a trpB gene that is monocistronic. Attempts to construct a trpB null mutant of Synechocystis by standard techniques were unsuccessful, suggesting that this organism is unable to concentrate tryptophan from the external medium.Keywords
This publication has 27 references indexed in Scilit:
- ACETYLORNITHINASE OF ESCHERICHIA COLI: PARTIAL PURIFICATION AND SOME PROPERTIESPublished by Elsevier ,2021
- An amino acid switch (Gly281–>Arg) within the “hinge” region of the tryptophan synthase beta subunit creates a novel cleavage site for the OmpT protease and selectively diminishes affinity toward a specific monoclonal antibodyJournal of Biological Chemistry, 1993
- Targeted inactivation of the gene psaL encoding a subunit of photosystem I of the cyanobacterium Synechocystis sp. PCC 6803Journal of Biological Chemistry, 1993
- Site-directed mutagenesis of the beta subunit of tryptophan synthase from Salmonella typhimurium. Role of active site glutamic acid 350.Journal of Biological Chemistry, 1991
- Genes for tryptophan biosynthesis in the archaebacterium Haloferax volcanii.Proceedings of the National Academy of Sciences, 1990
- Structure and targeted mutagenesis of the gene encoding 8-kDa subunit of photosystem I from the cyanobacterium Synechocystis sp. PCC 6803Journal of Biological Chemistry, 1989
- EVOLUTION OF A BIOSYNTHETIC PATHWAY: THE TRYPTOPHAN PARADIGMAnnual Review of Microbiology, 1989
- THE BETA-SUBUNIT OF TRYPTOPHAN SYNTHASE - CLARIFICATION OF THE ROLES OF HISTIDINE-86, LYSINE-87, ARGININE-148, CYSTEINE-170, AND CYSTEINE-2301989
- DNA sequence of the tryptophan synthase genes of Pseudomonas putidaBiochimie, 1989
- Structure of the Caulobacter crescentus trpFBA operonJournal of Bacteriology, 1988