Altered Growth of Human Colon Cancer Cell Lines Disrupted at Activated Ki- ras
- 2 April 1993
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 260 (5104) , 85-88
- https://doi.org/10.1126/science.8465203
Abstract
Point mutations that activate the Ki- ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki- ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki- ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c- myc . Thus, the activated Ki- ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.Keywords
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