THE TRANSPORT OF I125 POLY(I)-POLY(C) INCORPORATED INTO LYSOSOMES FROM THE ENTERIC CAVITY TO THE SMALL-INTESTINE MUCOSE - INVESTIGATION BY ELECTRON-MICROSCOPIC AUTORADIOGRAPHY

  • 1 January 1984
    • journal article
    • research article
    • Vol. 26  (7) , 783-+
Abstract
Transport of 125I-poly(I):poly(C) incorporated into liposomes through the small intestinal mucosa was investigated by EM autoradiography. With the migration of liposomes into the mucous layer on the luminal surface of the intestine up to the glycocalyx level of microvilli, these undergo degradation with the formation of monolayer liposomes from which the polynucleotide is released. Poly(I):poly(C) or its fragments are later transported through the enterocytes to be accumulated in cells of the connective tissue stroma of the small intestinal mucosa. Part of the polynucleotide is incorporated up to the arterial and lymphoid capillary level. Apparently, during transport the polynucleotide is affected by pancreatic and tissue nucleases. The accumulation of polynucleotide in macrophages, fibroblasts, lymphocytes, plasma cells and smooth muscle cells was traced. It is supposed that the polynucleotide accumulated in stroma of the small intestinal mucosa may preserve its interferon inducing activity.

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