The mass propagation of Lilium in vitro by stimulation of multiple adventitious bulb-scale formation and by shake culture

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Abstract
Multiple adventitious bulb scales of Lilium were investigated as a source for bulblet production. Addition of kinetin to the medium markedly increased the number of adventitious bulb scales differentiated from bulblets grown in vitro. The segments with numerous bulb scales were too small to separate into individual bulb scales, but the use of a shake culture technique markedly stimulated their growth. Such bulb scales were severed and each individual scale was transferred to fresh medium without kinetin to induce bulblet formation. The use of multiple adventitious bulb scales grown by shake culture seems to be advantageous for the mass propagation of Lilium bulblets.