A SIMPLE METHOD FOR THE ISOLATION OF PURINE NUCLEOSIDES FROM WORT AND BEER USING COLUMN CHROMATOGRAPHY
Open Access
- 2 January 1988
- journal article
- research article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 94 (1) , 33-37
- https://doi.org/10.1002/j.2050-0416.1988.tb04551.x
Abstract
Column chromatography using a variety of dextran based gel matrices was used to fractionate nucleosides in wort and beer. The gel matrices Sephadex G10 and Sephasorb HP Ultrafine were found to elute guanosine, deoxyguanosine, adenosine and deoxyadenosine in a single fraction whereas Sephadex LH20 and Sephadex G25 both yielded two fractions containing nucleosides. The first nucleoside containing fraction from Sephadex LH20 contained guanosine and the second fraction contained deoxyguanosine, adenosine and deoxyadenosine. In contract the first nucleoside containing fraction from Sephadex G25 contained both adenosine and deoxyadenosine and the second fraction contained guanosine and deoxyguanosine. Fractionation of low molecular weight components from wort and beer by column chromatography provides a simple technique for the isolation of purine nucleosides. This may be used to monitor directly the presence of purine nucleosides throughout the brewing process and to obtain quantitative estimates of the content of purine nucleosides in beer.Keywords
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