The Role of β, γ-Subunits of Guanine Nucleotide Binding Proteins in Control of a Reconstituted Signal Transmission Chain Containing Purified Components of the Adenylate Cyclase System
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in Journal of Receptor Research
- Vol. 7 (1-4) , 17-42
- https://doi.org/10.3109/10799898709054977
Abstract
The properties of a reconstituted signal transmission chain using purified β1-adrenoceptor (R), G-protein subunits (G) and adenylate cyclase (C) in lipid vesicles are described. This assay system was used to test β,γ-subunits of different origin with respect to their effects on R·G and R·G·C coupling and on the functional properties of Gsα and Giα. The findings reported here point to large differences in the efficacy of β,γ-subunits from different sources assessed by deactivation of [AlF4]- activated rabbit liver Gs and pertussis toxin-catalyzed ADP-ribosylation of bovine neutrophil Gα. This is explained by differences in the interaction domains of the interacting subunits. Furthermore, the sensitivity of R·G and R·G·C coupling to inhibition by β,γ-sub-units was greater than the effects of β,γ-subunits on hormonally activated GTPase activity of Gs. One of the consequences of differential inhibition of R·G·C coupling is an amplified response of the signal transmission chain to hormonal activation. This is in agreement with observations reported by Cerione et al. (1)Keywords
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