Hormonal Regulation of Pituitary Adrenocorticotrophin.

Abstract
A modification of the Saffran and Schally technique of in vitro bioassay of ACTH is described and evidence is presented for its precision, validity and reliability. Changes in pituitary ACTH content and concentration in rats after administration of various hormones for one week were studied by means of this technique. ACTH administration and adrenalectomy both resulted in a striking increase in pituitary ACTH content. Epinephrine and cortisone administration were both followed by significant depletion.