A RAPID METHOD FOR THE DETERMINATION OF DIASTATIC POWER
Open Access
- 2 January 1984
- journal article
- research article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 90 (1) , 37-39
- https://doi.org/10.1002/j.2050-0416.1984.tb04230.x
Abstract
A rapid method for the determination of diastatic power is described. The enzyme extraction and starch hydrolysis steps have been shortened to 10 min each and a colorimetric method involving the use of p-hydroxybenzoic acid hydrazide has been used for the determination of reducing sugars. The method avoids some sources of error associated with previous methods. The sensitivity of the method allows the use of small samples making it suitable for the measurement of diastatic power in barley breeding.Keywords
This publication has 9 references indexed in Scilit:
- Sensitivity of plant α-amylases to calcium ions in VitroPhytochemistry, 1981
- A simple colorimetric method for the determination of sugars in fruit and vegetablesJournal of the Science of Food and Agriculture, 1980
- Determination of Cereal Alpha Amylase Using a Commercially Available Dye‐labelled SubstrateStarch ‐ Stärke, 1974
- AUTOMATIC ESTIMATION OF MALT DIASTATIC ACTIVITYJournal of the Institute of Brewing, 1973
- A new reaction for colorimetric determination of carbohydratesAnalytical Biochemistry, 1972
- INSTITUTE OF BREWING ANALYSIS COMMITTEE COLLABORATIVE MALT ANALYSESJournal of the Institute of Brewing, 1970
- RECOMMENDED METHODS OF ANALYSIS OF BARLEY, MALT AND ADJUNCTS ALTERNATIVE TITRATION PROCEDURE FOR THE DETERMINATION OF THE DIASTATIC ACTIVITY OF MALTJournal of the Institute of Brewing, 1970
- MODIFIED PROCEDURE FOR THE DETERMINATION OF DIASTATIC ACTIVITY AND α-AMYLASE ACTIVITYJournal of the Institute of Brewing, 1963
- Use of Dinitrosalicylic Acid Reagent for Determination of Reducing SugarAnalytical Chemistry, 1959