Iron Complexes and Their Reactivity in the Bleomycin Assay for Radical-Promoting Loosely-Bound Iron
- 1 January 1986
- journal article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 2 (3) , 143-151
- https://doi.org/10.3109/10715768609088066
Abstract
The sensitivity of the bleomycin assay for loosely-bound iron depends on the concentration of bleomycin and ascorbic acid and the pH of the reaction. The non-haem-iron proteins transferrin, conalbumin and ferritin release iron at an acid pH value, whereas the haem-iron proteins release iron more readily at an alkaline pH. In addition, haem proteins are liable to release iron when peroxides are present. Organic peroxides and hydrogen peroxide can be produced during the bleomycin reaction leading to iron release from haem proteins. However, this can be prevented from reacting with bleomycin by adding zinc ions to the reaction following addition of the sample. Iron already bound to bleomycin is not displaced by zinc whereas zinc bound to bleomycin is not displaced by iron allowing 'free' and 'released' iron to be discriminated.Keywords
This publication has 13 references indexed in Scilit:
- Antioxidant properties of the proteins caeruloplasmin, albumin and transferrin. A study of their activity in serum and synovial fluid from patients with rheumatoid arthritisBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1986
- Structure of the alkali-labile product formed during iron(II)-bleomycin-mediated DNA strand scissionJournal of the American Chemical Society, 1985
- Low-molecular-weight iron complexes and oxygen radical reactions in idiopathic haemochromatosisClinical Science, 1985
- Copper and iron complexes catalytic for oxygen radical reactions in sweat from human athletesClinica Chimica Acta; International Journal of Clinical Chemistry, 1985
- Lipid peroxidation in rheumatoid arthritis: Thiobarbituric acid-reactive material and catalytic iron salts in synovial fluid from rheumatoid patientsClinical Science, 1984
- INCREASED NON-PROTEIN-BOUND IRON AND DECREASED PROTECTION AGAINST SUPEROXIDE-RADICAL DAMAGE IN CEREBROSPINAL FLUID FROM PATIENTS WITH NEURONAL CEROID LIPOFUSCINOSESThe Lancet, 1982
- Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of ‘free’ iron in biological systems by using bleomycin-dependent degradation of DNABiochemical Journal, 1981
- Inhibition of lipid peroxidation by the iron-binding protein lactoferrinBiochemical Journal, 1981
- Intermediates in the ferrous oxidase cycle of bleomycinBiochemistry, 1981
- Iron-dioxygen-dependent changes to the biological activities of bleomycinJournal of Inorganic Biochemistry, 1981