Differential effects of colchicine and cytochalasins on the shedding of murine B cell membrane IgM and IgD.

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Abstract
Lactoperoxidase (EC 1.11.1.7) catalyzed cell surface radioiodination was employed to monitor the fate of murine B [bone marrow-derived] cell membrane (mem) Ig[immunoglobulin]M and IgD on radiolabeled cells in short-term culture. Both mem-IgM and mem-IgD were shed from cell surface with biphasic kinetics. The rapid phase of mem-IgD shedding was somewhat slower (half-time = 12 h) than that of mem-IgM shedding (half-time = 7-8 h). The effect of temperature, colchicine and cytochalasin on the shedding of the 2 membrane Ig isotypes was determined. Shedding of mem-IgD was more energy dependent than that of mem-IgM and was sensitive to colchicine but not cytochalasin. Conversely, the shedding of mem-IgM was sensitive to cytochalasin but not colchicine. The mechanisms of shedding of mem-IgM and mem-IgD are qualitatively distinct and may be regulated by microfilaments or microtubules, respectively.

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