Granular cell myoblastoma: an immunoperoxidase study using a variety of antisera to human carcinoembroyonic antigen

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Abstract
Immunoperoxidase staining using 5 antisera to human carcinoembryonic antigen (CEA), including a mouse monoclonal antibody, was performed to investigate the expression of CEA reactivity in 10 cases of oral granular cell myoblastoma. The granular cells were negative with 4 of the antisera although control sections of CEA producing colon carcinoma were positive. The single positive antiserum gave intense granular cytoplasmic staining of all tumor cells in the 10 specimens studied. This reactivity was abolished after absorption of the antiserum with a perchloric acid extract of human lung to remove cross-reacting antibodies against non-specific cross-reacting antigen, a procedure which did not affect the staining of colon carcinoma specimens. The granular cells do not contain CEA but express a related antigen. Care in the choice of primary antiserum is important if the immunocytochemical detection of this antigen is to be used as a diagnostic aid.