BIOCHEMICAL STUDIES ON THE PHENOMENON OF VIRUS REPRODUCTION

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Abstract
A method for the study of the requirements of the virus system was proposed and tested. The method calls for the detn. of an activity ratio for an inhibitor, which indicates how much more of the inhibitor is required to delay the growth of the uninfected host cells than to inhibit phage multiplication. Inhibitions of a virus-host system by salicylate and gentisate in a synthetic medium were studied as examples of the method. The activity ratio for salicylate was 6 to 10. The activity ratio for gentisate was 100. DL-tryptophan, indole or anthranilic acid prevented salicylate inhibition of phage multiplication, but not that of bacterial growth, whereas pantothenate partially prevented the host cell growth inhibition. L-tyrosine prevented both phage and bacterial growth inhibitions by gentisate. Inhibition of phage multiplication by old solns. of gentisate was reversed also by DL-methionine and ribonucleic acid.