Das Salicylat-Ion als spezifischer Hemmungsstoft der Biosynthese der Pantothensäure

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Abstract
The growth of a thin suspension of a 24-hr -old culture of Staphylococcus aurens in 4 ml. casein nutrient medium, in which the conc. of Ca pantothenate was 1/0.83 [center dot] 10[degree]-l/2.5 [center dot] 107 mol and the conc. of Na salicylate was A//62-M/500, was detd. The vol. of the mixture was adjusted to 5 ml. with water, the mixture placed in the thermostat at 37[degree] for 24 hrs., and the amt. of growth detd. with a nephelometer. The growth of the Staphylococcus, which is able to synthesize pantothenic acid, was prevented by the salicylate. This effect of the salicylate was destroyed by pantothenic acid, or the a-hydroxy-[beta]-[beta]-dimethyl-[gamma]-butyrllactone component of pantothenic acid but was not affected by the alanine component. Proteus morganii was grown on a modified Pelczar and Porter nutrient medium (J. Biol. Chem., 139: 110, 1941). 9 ml. of the medium and varying amts. of Na salicylate and Ca pantothenate were placed in tubes and the vol. adjusted to 10 ml. with water. The tubes were inoculated with a 24-hr. culture of P. morganii. The conc. of pantothenate was M/2 . 109-M/2 . 106, and of salicylate, M/1000-M/40. Growth of P. morganii was prevented by M/40 salicylate, and the action of the salicylate was little affected by the amt. of pantothenic acid present. P. morganii does not synthesize pantothenic acid. When no salicylate was present, pantothenic acid and its lactone stimulated growth of the bacillus. The medium used for the Escherichia coli was that used by Sahyan et al. (Jour. Infect. Dis., 58: 28, 1936) with some modifications. Growth of the bacillus was prevented when the salicylate conc., was M/5 . 104. This action of the salicylate was prevented by the addition of pantothenic acid to the medium. E. coli synthesizes pantothenic acid.