Titration of a Vaccine Stock Preparation of Human Immunodeficiency Virus Type 1sf2in Cultured Lymphocytes and in Chimpanzees

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Abstract
A large stock preparation of the HIV-1sf2 isolate has been derived after serial passage in human peripheral blood mononuclear cells (PBMCs). This viral stock has a titer of 104.9 TCID50 in human PBMCs and 104.2 TCID50 in chimpanzee PBMCs. By inoculation into animals the 50% chimpanzee infectious dose titer was found to be about 102.3. Virus isolation from animals was achieved on most occasions within 1–4 weeks after inoculation and then became transient. Viral RNA and DNA PCR analyses confirmed the virus infection of the chimpanzees. Anti-HIV antibody levels in the inoculated animals ranged from 1:400 to 1:6400 as measured by ELISA. About 680 vials of this stock preparation, frozen at -190°C, are available for future studies of vaccines and antiviral therapies.