Some Factors Affecting the Clearing of Neutral Fat Emulsion by Post-Heparin Plasma

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Abstract
A method to determine the clearing of a synthetic neutral fat (triglycerides) emulsion in vitro by the lipemia clearing factor of dogs'' post-heparin plasma is described. The quantity of lipemia clearing factor produced in vivo depends on the dose of heparin injected. The relationship is linear between 0.0-0.05 mg of heparin/kg of body weight. With administration of larger doses of heparin, the clearing activity in vivo persisted longer but without significant increase in the magnitude of clearing. The clearing factor of post-heparin plasma of dogs is thermo-labile, but relatively stable at 0[degree]-5[degree]C. The properties of clearing reaction are: (a) optimal pH, 6.4-7.0; (b) optimal temperature, 35[degree]-40[degree]C; (c) higher initial clearing rate during the course of incubation; (d) optimal substrate concentration: neutral fat emulsion, 0.32-0.72 mg/ml; lymph fat, 0.48-1.0 mg/ml; and (e) clearing activity increased by the addition of sodium acetate.

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