ANTISERA AGAINST LEUKAEMIA-ASSOCIATED ANTIGENS ON HUMAN LYMPHOCYTES

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 27  (3) , 487-496
Abstract
Antisera were raised in rabbits against leukemic lymphosarcoma (LSL) cells which carried surface markers of thymus-derived (T) lymphocytes and bone marrow-derived (B) lymphocytes. After absorption with leukocytes, erythrocytes and serum proteins from normal individuals, the antisera demonstrated significant complement-dependent cytotoxicity against leukemic cells from patients with acute lymphoblastic leukemia (ALL) (9/11), LSL (7/9) and chronic lymphocytic leukemia (CLL) (9/12), with an antibody titer of 1:64 or greater. The antisera did not react with: blood lymphocytes from clinically healthy individuals (0/23), patients with non-lymphoproliferative disorders (0/8) and normal umbilical cords (0/3); normal lymphocytes stimulated by pokeweed mitogen (0/7), allogeneic lymphocytes (0/3), fetuin (0/1), purified protein derivative (PPD) (0/2), and Candida antigen (0/1); normal marrow cells (0/3); normal thymocytes (0/2); and leukemic cells from patients with acute myeloblastic (AML) (0/10) and chronic granulocytic leukemia (CGL) (0/3). The antisera did react with lymphoblastoid cells from continuous B-cell lines derived from an AML patient and from a non-leukemic individual and, to a lesser extent, with lymphocytes from patients with infectious mononucleosis. The antisera also reacted with lymphocytes from chronically infected tonsils. Cytotoxicity of the antisera against lymphoblastoid and tonsillar cells was inhibited by ALL and CLL cell-lysates; conversely, cytotoxicity against ALL cells was inhibited by the lymphoblastoid cell extract. A cell lysate or extract from normal lymphocytes did not inhibit cytotoxicity toward lymphoblastoid, tonsillar or ALL cells. Cytotoxicity of the antisera was neutralized by a goat anti-rabbit Ig[immunoglobulin]G. The antisera apparently contained antibodies reactive with antigens possibly common to neoplastic lymphocytes, tonsillar cells, lymphoblastoid cells and some blood lymphocytes from patients with infectious mononucleosis.