The chemistry of plant cuticles: a study of cutin from Agave americana L

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Abstract
The ether-soluble fraction, obtained on saponifi-cation of cutin from Agave americana, was resolved by means of counter-current distribution and reversed-phase partition chromatography, the latter method being adapted to a preparative scale. Four acids, namely threo-9,10,18-trihydroxyoctadecanoic, 10,18-dihydroxyoctadecanoic, 18-hydroxyoctadecanoic and 10,16-dihydroxyhexadecanoic acid, were isolated in a pure state. The 5th, 18-hydroxyoctadec-cis-9-enoic acid, was obtained as its dihydroxy derivative. The nature of cutin from A. americana and its similarity to certain other natural products is discussed.
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