Electrophoretic Patterns of Oxidoreductases and Other Proteins As Criteria in Fungal Taxonomy
- 1 January 1968
- journal article
- research article
- Published by CSIRO Publishing in Australian Journal of Biological Sciences
- Vol. 21 (2) , 275-296
- https://doi.org/10.1071/bi9680275
Abstract
Oxidoreductases and other proteins were extracted from fungi and separated by electrophoresis in starch gels. Isolates of FU8arium, Phytophthora, Pythium, Saccharomyces, Schizosaccharomyces, and Thanatephorus (Rhizoctonia) were used. The main soluble proteins were detected by staining with amido black after electrophoresis in starch gel with a discontinuouA citrate-borate system in the pH range 8·2-9·5. Alcohol dehydrogenase (EC 1.1.1.1.), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), malate dehydrogenase (EC 1.1.1.37), diaphorase (i.e. various NADH2-nitro blue tetrazolium oxidoreductases) andKeywords
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