Immobilization of fumarase by entrapment of rat liver mitochondria in polyacrylamide gel using gamma rays
- 1 January 1983
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 25 (1) , 217-224
- https://doi.org/10.1002/bit.260250117
Abstract
A stable immobilized preparation of fumarase (EC 4.2.1.2) was obtained by entrapment of rat liver mitochondria in acrylamide polymerized by using gamma irradiation (100 kR). The enhanced stability and the efficiency of the entrapped enzyme have shown potential for repeated use for the production of L‐malic acid from fumaric acid. The possible formation of succinic acid in the system could be controlled by incorporating malonate along with detergents such as sodium deoxycholate or sodium dodecylsulfate in the reactor system.This publication has 18 references indexed in Scilit:
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