HYBRIDIZATION BETWEEN THE NUCLEAR AND KINETOPLAST DNA'S OF Leishmania enriettii AND BETWEEN NUCLEAR AND MITOCHONDRIAL DNA'S OF MOUSE LIVER

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Abstract
Two methods were used to study the degree of hybridization between nuclear and kinetoplast DNA''s of L. enriettii and nuclear and mitochondrial DNA''s of mouse liver. The first method involved the use of the DNA-agar technique under conditions favoring high specificity of binding, i.e., elevated temperature. In this case, labeled nuclear fragments competed for sites on nuclear DNA-agar with either unlabeled nuclear or extranuclear DNA fragments. The 2nd method involved the use of membrane filters with the improvements proposed by Denhardt. Here the degree of binding of labeled nuclear fragments to either membrane-immobilized nuclear or extranuclear DNA was studied. The results obtained by either method indicate that the extranuclear DNA''s contain nucleotide sequences, similar enough to nuclear DNA fragments to allow labeled nuclear DNA fragments to reassociate 40-70% as effectively with extra-nuclear DNA as with the corresponding nuclear DNA.

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